Figure 2. Lipid deposition in submandibular gland and lipogenesis analysis. (A) Lipid vacuoles (yellow circle) of submandibular gland detected by H & E staining. (B) Morphometric analysis of lipid vacuoles in the CON and OVX groups. (C), (D) Real time PCR and western blots of lipogenesis –related gene; ACC alpha and transcription factors; SREBP-1C and ChREBP expression in the CON and OVX groups. Two-way ANOVA test. *p<0.05, **p<0.01 and ***p<0.001. CON = control, OVX = ovariectomy, ACC = Acetyl-CoA carboxylase, SREBP = sterol regulatory element-binding protein, ChREBP = carbohydrate response element-binding protein.