Research Paper Volume 12, Issue 21 pp 21376—21390

The mechanism of submandibular gland dysfunction after menopause may be associated with the ferroptosis

Figure 2. Lipid deposition in submandibular gland and lipogenesis analysis. (A) Lipid vacuoles (yellow circle) of submandibular gland detected by H & E staining. (B) Morphometric analysis of lipid vacuoles in the CON and OVX groups. (C), (D) Real time PCR and western blots of lipogenesis –related gene; ACC alpha and transcription factors; SREBP-1C and ChREBP expression in the CON and OVX groups. Two-way ANOVA test. *p<0.05, **p<0.01 and ***p<0.001. CON = control, OVX = ovariectomy, ACC = Acetyl-CoA carboxylase, SREBP = sterol regulatory element-binding protein, ChREBP = carbohydrate response element-binding protein.