Research Paper Volume 12, Issue 22 pp 22700—22718

Drug-selected population in melanoma A2058 cells as melanoma stem-like cells retained angiogenic features – the potential roles of heparan-sulfate binding ANGPTL4 protein

Figure 7. Effects of suppression of ANGPTL4 expression on melanoma-stem marker expression and phosphor-protein expressions in drug-selected cells. (A) Effect of the ANGPTL4 suppression on expression of melanoma-stem markers in drug-selected cells as analyzed by qPCR. (B) Effect of the ANGPTL4 suppression on expression of phosphor-tyrosine proteins in drug-selected cells as analyzed by western blot analysis. Significantly higher levels of phosphor-proteins in drug-selected cells were indicated by arrows. Line-histograms were generated by Image J.