Research Paper Volume 12, Issue 22 pp 22719—22743

Gonadal white adipose tissue-derived exosomal MiR-222 promotes obesity-associated insulin resistance

Figure 2. Gonadal white adipose tissue is a major source of serum exosomal miR-222. (A) QRT-PCR analysis shows the miR-222 levels in the exosome (n=5; blue portion of the bar) and exosome-free (n=5; white portion of the bar) fractions of the wild-type mouse serum. (B) The data from the GitHub database shows the miR-222 levels in the serum exosomes from the wild-type (black bar) and ADicerKO (adipose-tissue-specific knockout of Dicer; red bar) mice (n=4/group). (C) The GSE81976 dataset analysis shows the miR-222 levels in the brown adipose tissue (BAT; black bar; n=1), inguinal white adipose tissue (iWAT; red bar; n=1) and the gonadal white adipose tissue (gWAT; blue bar; n=1) of chow-fed mice. (D) The GSE85101 dataset analysis shows the miR-222 levels in the brown adipose tissue (black bar; n=3), inguinal white adipose tissue (red bar; n=3) and gonadal white adipose tissue (blue bar; n=3) from the HFD-fed mice. (E) The GitHub database analysis shows the levels of miR-222 in the serum exosomes from wild-type mice after sham surgery (n=3), ADicerKO mice after sham surgery (n=4), and ADicerKO mice after transplantation with wild-type-derived gonadal WAT (n=4). Note: The data are presented as the means ± SE; * P < 0.05, ** P < 0.01, *** P < 0.001.