Research Paper Volume 12, Issue 22 pp 22719—22743

Gonadal white adipose tissue-derived exosomal MiR-222 promotes obesity-associated insulin resistance

Figure 3. Gonadal adipose tissues are the major source of the elevated miR-222 in the serum and serum exosomes of high-fat diet (HFD)-induced obese model mice. (AC) QRT-PCR analysis shows the relative miR-222 levels in the (A) serum, (B) serum exosomes, and (C) gonadal white adipose tissues (gWAT) harvested from the male CD-fed or HFD-fed mice (n=5 per group). (D) QRT-PCR analysis shows the relative levels of pri-miR-222 in the heart, liver, spleen, lung, kidney, brain, skeletal muscle and gonadal adipose tissue harvested from the male CD-fed or HFD-fed mice (n=5 per group). (E, F) QRT-PCR analysis shows the relative expression of miR-222 in the (E) in-vitro cultured gWAT tissue slices from CD-fed and HFD-fed mice (n=5 per group) and (F) exosomes from the culture medium of the in vitro cultured gWAT tissue slices from the CD-fed and HFD-fed mice (n=6 per group). The samples were extracted after in vitro culturing for 48h. (G, H) The relative levels of miR-222 in the (G) serum and (H) serum exosomes from gWAT-lipectomized (gWL) and sham-operated (Sham) HFD mice (n=8 per group). Note: The data are presented as the means ± SE. * P < 0.05, ** P < 0.01, *** P < 0.001.