Research Paper Volume 12, Issue 23 pp 23698—23726

The ZiBuPiYin recipe regulates proteomic alterations in brain mitochondria-associated ER membranes caused by chronic psychological stress exposure: Implications for cognitive decline in Zucker diabetic fatty rats


Figure 10. Western blotting analysis for selected differentially expressed proteins. MAM fractions from ZDF, PSD, and PDZ rats were analyzed by western blotting using antibodies against mitogen-activated protein kinase 1 (Mapk1) (A), apolipoprotein E (Apoe) (B), pyruvate kinase (Pkm) (C), glyceraldehyde-3-phosphate dehydrogenase (Gapdh) (D), and ALG2, alpha-1,3/1,6-mannosyltransferase (Alg2) (E). VDAC1 was used to ensure equal protein loading and transfer. The levels of Pkm, Mapk1, Apoe, Gapdh, and Alg2 were normalized relative to VDAC1 levels. *p <0.05, ***p <0.001, ****p <0.0001 PSD vs. ZDF; #p <0.05, ##p <0.01, ####p <0.0001 (mean ± SEM, n=3 per group).