Figure 9. High-Pi diet alters Nrf2 and p62 expression in GA muscle from sham-operated and CKD mice. Eight-week-old mice underwent either a sham operation or 5/6 nephrectomy (CKD), after which they were fed a normal Pi (NP) or high-Pi (HP) diet for 20 weeks. Cytosolic (A and C) and nuclear (B and D) fractions were extracted from GA muscle samples and probed for Nrf2 and p-Nrf2 (A and B) and for p62 and p-p62 (C and D). Levels of these proteins were quantified by computer-assisted densitometric analysis. (E) Representative images of p-Nrf2 immunohistochemistry in GA muscle sections. (F) Semiquantitative IHC scoring from images like those shown in (E). Data are presented as means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.