Figure 7.Inhibition of PPARγ abolished the effect of GE. LPS-induced HK-2 cells was utilized to simulate sepsis-induced kidney injury. Cells were treated with GE (100 μg/ml, 200 μg/ml, 300 μg/ml), and the mRNA level of PPARγ was detected by qRT-PCR (A). GW9662, a PPARγ antagonist, was employed to treat HK-2 cells, and then the concentrations of inflammatory cytokines including TNF-α, IL-6, IL-10, IL-1β, and MCP-1 in each group were measured using ELISA kit (B–F). Cell apoptotic rate in each group was determined via usage of flow cytometry analysis (G, H). **, ***p<0.01, 0.001 vs the control group. ##, ###p<0.01, LPS $$, $$$ p<0.01, LPS+GE.
