Research Paper Volume 12, Issue 22 pp 22759—22775

NEAT1/miR-200b-3p/SMAD2 axis promotes progression of melanoma

Figure 6. NEAT1 regulated tumor proliferation and invasion through miR-200b-3p. (A) RT-qPCR was used to test the efficiency of NEAT1 overexpression. (B) RT-qPCR was used to detect the expression of miR-200b-3p after cells were transfected with LV-NEAT1 and/or miR-200b-3p mimic. (CD) Cell proliferation was measured by flow cytometry and CCK8 after cells were transfected with LV-NEAT1, LV-NEAT1+miR-200b-3p mimic or control (the OD value was measured at 0h, 24h, 48h and 72h). (EF) Cell invasion was detected after cells were transfected with LV-NEAT1, LV-NEAT1+ miR-200b-3p mimic or control at 12h. (G) Tumor volume was calculated after injection of A375 cells transfected with LV-NEAT1, LV-NEAT1+ miR-200b-3p mimic or control (n=8).