Research Paper Volume 12, Issue 22 pp 22759—22775

NEAT1/miR-200b-3p/SMAD2 axis promotes progression of melanoma

Figure 8. The NEAT1/miR-200b-3p/SMAD2 axis tended to promote melanoma by immune regulation. (A) Pan-cancer analysis was performed to test the positive rates of SMAD2 in several cancer tissues. (B) Pan-cancer analysis showed that SMAD2 was significantly increased In 17 types of cancer tissues. (C) Sequencing data and clinical characteristic was analyzed to show SMAD2 related with signaling pathways. (D) Western blot determined SMAD2-associated signaling proteins after transfection with LV-miR-200b-3p in A375 and M14 cells. (E) Kaplan-Meier plots was drawn to visualize the survival outcomes for different immune infiltrates according to a multivariable Cox proportional hazard model. (F) Immune infiltrates assays demonstrated SMAD2-related immune cells after melanoma patients were classified according to SMAD2 levels.