Research Paper Volume 12, Issue 21 pp 21469—21480

The protective effects of memantine against inflammation and impairment of endothelial tube formation induced by oxygen-glucose deprivation/reperfusion

Figure 8. The effects of memantine on endothelial microtubule formation are mediated by the PI3K/Akt signaling in HUVECs. (A) Cells were treated with memantine (10 μM) for 6 h, followed by exposure to oxygen-glucose deprivation (6 h)/reperfusion (24 h) (OGD/R). Phosphorylated and total levels of PI3K and Akt were measured; (B) Inhibition of PI3K/Akt with LY294002 (20 μM) abolished the protective effects of memantine in microtubule formation (####, P<0.0001 vs. vehicle group; *****, P<0.0001 vs OGD/R group; $$$$, P<0.0001 vs. OGD/R+ memantine group).