Research Paper Volume 12, Issue 20 pp 20561—20586

Colorectal cancer patients with CASK promotor heterogeneous and homogeneous methylation display different prognosis

Figure 2. Direct sequencing for methylation-sensitive high-resolution melting (MS-HRM) product of sample TN07221. Primers are marked in yellow, black arrows indicate CpG sites with overlapping peak. (Dye blobs in the unidirectional Sanger sequencing result in uncertainty of bases at the beginning of sequence giving incomplete coverage. Hence, we only provided the definite part of the entire sequencing result.)