Figure 1. Flow chart of the present study. This study was designed as follows: rat brain tissues of ICH and sham were collected and then altered expressed tsRNAs with altered expression were obtained by RNA-Sequencing. Subsequently, we predicted and validated the mRNA targets of ICH-responsive tsRNAs. Furthermore, we evaluated the potential tsRNA functions post-ICH by bioinformatics tools. Finally, the bioinformatics results were validated in vitro.