Figure 3. The validation of ICH-responsive tsRNAs. (A) The tsRNA-Seq data revealed 12 significantly changed tsRNAs. The 12 significantly changed tsRNAs are shown in the heat map (the left part of A). The expression levels of the 12 tsRNAs by RNA-sequencing data are exhibited in the bar chart (the right part of A). (B) The qRT-PCR results showed that among the 12 tsRNAs, only 7 tsRNAs were significantly changed. The 7 tsRNAs were confirmed as the actual ICH-responsive tsRNAs. Data are presented as the mean ± SEM, (n=3 each group in A, and n=6 each group in B), *P<0.05, ** P<0.01, *** P<0.001. TPM indicates tag counts per million of total aligned tRNA reads, representing the tsRNA expression levels.