Figure 3. Differential gut microbial profiles of non-septic and septic mice after light exposure. (A) Treatment schedule. Mice were treated with or without LPS (20 mg/kg), and then were exposed to light for seven days. Fresh feces were collected on days 1, 3 and 7 for 16S rRNA gene sequencing. (B) Heat map of the fecal bacterial abundances in the different groups. LPS administration and red light exposure reduced the richness of the intestinal flora and altered the gut microbial composition, as determined using the Chao 1 index (C), Shannon index (D), ACE index (E), Simpson index (F) and 3D principal coordinate analysis (G). Data are presented as the mean ± SEM (n = 6-7/group). *P < 0.05, **P < 0.01, ***P < 0.001, #P < 0.05, ##P < 0.01.