Figure 6. Experimental validation of IGF2BP2 over-expression in PAAD tissue array and cell lines in vitro. (A) Immunohistochemistry staining (IHC) for a pair of PAAD tissues and their adjacent normal tissues for IGF2BP2 protein. The upper part of the figure showed normal pancreas tissues and the lower part showed the corresponding PAAD tissue staining. The two pictures in left panel were magnified 200 times (200x), and the right panel, 400 times (400x). (B) Quantitative real-time PCR (qRT-PCR) for mRNA expression of IGF2BP2 in 20 pairs of PAAD cells and their corresponding normal pancreatic cells. (C) qRT-PCR of IGF2BP2 for 4 PAAD cell lines and one normal pancreatic ductal epithelial cell line (HPDE) in vitro. (D) Western Blotting (WB) showed the protein level of IGF2BP2 in 4 PAAD cell lines and HPDE cell line in vitro (**, P < 0.01; ***, P < 0.001).