Figure 3. Hsa_circ_0081534 bound to miR-508-5p. (A, B) Subcellular fractionation assays were utilized to test for localization of hsa_circ_0081534. (C, D) A Venn diagram showing predicted binding sites between hsa_circ_0081534 and miR-508-5p. (E) Pull down assay showing that miR-508-5p was abundantly pulled-down by hsa_circ_0081534 probe. (F) Relative luciferase activity in NPC cells was measured by dual-luciferase reporter assays. (G) RIP assay facilitated the enrichment of hsa_circ_0081534 and miR-508-5p in NPC cells. *P < 0.05.