Research Paper Volume 12, Issue 20 pp 20658—20683

Cross-talk between the ER pathway and the lncRNA MAFG-AS1/miR-339-5p/ CDK2 axis promotes progression of ER+ breast cancer and confers tamoxifen resistance

Figure 2. lncRNA MAFG-AS1 promotes the development of ER+ breast cancer through cell cycle and apoptosis. (A, B) Knockdown of MAFG-AS1 significantly decreased cell proliferation compared with si-NC cells in T47D and MCF-7 cell lines by MTT assay. (C) Colony formation number of T47D and MCF-7 cells transfected with si-MAFG-AS1 were significantly less than those transfected with si-NC. (D) MAFG-AS1 promoted cell cycle from G1 to S phase by flow cytometry. (E) MAFG-AS1 promoted antiapoptosis by Annexin-V/FITC double staining on cell apoptosis; the bar graph presents the percentage of apoptotic cells. (F) Tumor images of MCF-7-shNC and MCF-7-shMAFG-AS1 cells which were infected with lentiviruses carrying the indicated expression constructs injected into female nude mice pad for in situ tumor formation. (G) Tumor volume (left) and weight (right) in the sh-MAFG-AS1 group were significantly lower than those in the sh-NC group. (H, I) Colony formation of E2 combined with or without knocking down MAFG-AS1. Data are presented as the mean±SD of three independent experiments. *p<0.05.**p<0.01.