Research Paper Volume 12, Issue 21 pp 21674—21686

Circular RNA circSEMA5A promotes bladder cancer progression by upregulating ENO1 and SEMA5A expression

Figure 2. CircSEMA5A accelerates the oncogenic process of BC cells. (AJ) T24 cells were transfected with circSEMA5A overexpression plasmids and UM-UC-3 cells were transfected with circSEMA5A siRNAs. (A and B) Cell proliferation was measured by EdU assays; (C and D) cell apoptosis was detected by flow cytometry; (E and F) cell migration was assessed using wound-healing assays; (G and H) cell invasion was evaluated by transwell assays; (I and J) angiogenesis capability was assessed by tube formation assay. (K) Xenograft assay assessing the effect of circSEMA5A knockdown on tumor growth in vivo. Data are presented as mean ± SD. *P < 0.05.