Research Paper Volume 12, Issue 19 pp 18907—18927

Prolonged oxidative stress and delayed tissue repair exacerbate acetaminophen-induced liver injury in aged mice

Figure 3. Defective glutathione synthesis in aged livers after APAP injury. (A) GSH oxidative stress is sustained in aged mice. Serum GSH is reduced at 8 hours after APAP administration in both young and aged mice. GSH levels are back to normal levels at 24 hours in young but not in aged mice. The graph shows average values with SEMs. (B) Expression of glutamate-cysteine ligase (GCL). Gclc, GCL catalytic subunit, is significantly upregulated at 8 and 24 hours after APAP administration in young mice. Gclm, the GCL modifier subunit, tends to be upregulated at 24 hours. By contrast, both Gclc and Gclm are not upregulated in aged mice. The graph shows average values with SEMs.