Research Paper Volume 12, Issue 21 pp 21687—21705

Dexmedetomidine inhibits inflammatory response and autophagy through the circLrp1b/miR-27a-3p/Dram2 pathway in a rat model of traumatic brain injury

Figure 7. Knockdown of Dram2 enhances the suppressive effects of miR-27a-3p in traumatic brain injury-induced neurological outcome, autophagy, and inflammation. Rats were administered intracerebroventricular injection of lentivirus vectors of miR-27a-3p mimics and sh-Dram2 before traumatic brain injury (TBI) induction. (A) Calculation of the brain water content, as described in the Materials and Methods section. (B) Analysis of the modified Neurological Severity Score (mNSS). (C) Expression levels of circLrp1b, miR-27a-3p, and Dram2, as determined by real-time quantitative reverse transcriptase polymerase chain reaction. (D) Expression levels of Dram2, ATG5, Beclin-1, p62, LC3 I/II, caspase-1, and NLRP3 proteins, as measured using western blot. Quantitative analysis of enzyme-linked immunosorbent assay (ELISA) detection of TNF-α (E), IL-6 (F), and IL-1β (G) production in the hippocampal tissues. Each experiment was repeated 6 times. **p < 0.01, ***p < 0.001, compared with Sham; #p < 0.05, ##p < 0.01, compared with TBI + miR-NC; &p < 0.05, compared with TBI + mimics + sh-NC.