Research Paper Volume 12, Issue 21 pp 21706—21729

Puerarin alleviates osteoporosis in the ovariectomy-induced mice by suppressing osteoclastogenesis via inhibition of TRAF6/ROS-dependent MAPK/NF-κB signaling pathways

Figure 3. Puerarin treatment reduces ROS levels in the bone tissues of the OVX-induced osteoporosis model mice. (A, B) Fluorescence images show the ROS levels based on DHE staining in the cryosections of femur bone tissues from the sham, OVX+vehicle, and OVX+puerarin mice (n=5 per group). Note: **P <0.01 vs. the OVX+vehicle group. (CE) Representative images show the IHC staining of the femur bone sections from the sham, OVX+vehicle, and OVX+puerarin mice with antibodies against NOX1 and HO-1. Also shown is the quantitative analysis of NOX1 and HO-1 expression in the bone sections from the sham, OVX+vehicle, and OVX+puerarin mice (n=5 per group). Note: **P <0.01 vs. the OVX+vehicle group.