Research Paper Volume 13, Issue 2 pp 1883—1897

CD36 upregulates DEK transcription and promotes cell migration and invasion via GSK-3β/β-catenin-mediated epithelial-to-mesenchymal transition in gastric cancer

CD36 promotes migration and invasion in GC cells. (A) Analysis of CD36 protein expression in GC cell lines. (B) Analysis of CD36 mRNA expression in GC cell lines. (C) Flow cytometric analysis of the transfection efficiencies of NC/siCD36 in SNU-216 cells. (D) Western blot analysis of the transfection efficiencies of NC/siCD36 in SNU-216 cells. (E) Results of Transwell migration and invasion assays in SNU-216 cells transfected with NC/siCD36. (F) Flow cytometric analysis of the transfection efficiencies of NC/pcDNA3.1-CD36 in MKN-45 cells. (G) Western blot analysis of the transfection efficiencies of NC/pcDNA3.1-CD36 in MKN-45 cells. (H) Results of Transwell migration and invasion assays in MKN-45 cells transfected with NC/pcDNA3.1-CD36. Data are presented as the mean ± SD. *p

Figure 2. CD36 promotes migration and invasion in GC cells. (A) Analysis of CD36 protein expression in GC cell lines. (B) Analysis of CD36 mRNA expression in GC cell lines. (C) Flow cytometric analysis of the transfection efficiencies of NC/siCD36 in SNU-216 cells. (D) Western blot analysis of the transfection efficiencies of NC/siCD36 in SNU-216 cells. (E) Results of Transwell migration and invasion assays in SNU-216 cells transfected with NC/siCD36. (F) Flow cytometric analysis of the transfection efficiencies of NC/pcDNA3.1-CD36 in MKN-45 cells. (G) Western blot analysis of the transfection efficiencies of NC/pcDNA3.1-CD36 in MKN-45 cells. (H) Results of Transwell migration and invasion assays in MKN-45 cells transfected with NC/pcDNA3.1-CD36. Data are presented as the mean ± SD. *p < 0.05 vs. NC group. GAPDH was used as endogenous control in western blot assays. IDV: integrated densitometric value.