Research Paper Volume 12, Issue 19 pp 19660—19676
Anticancer effects of miR-124 delivered by BM-MSC derived exosomes on cell proliferation, epithelial mesenchymal transition, and chemotherapy sensitivity of pancreatic cancer cells
Exosomes were extracted from BM-MSCs and delivered miR-124 into pancreatic cancers. The BM-MSCs were identified using flow cytometry (A) since CD90 and CD105 were positive, and CD34 and CD45 were negative. The extracted exosomes were examined by transmission electron microscopy (B) The expression level of miR-124 in BM-MSCs were determined by RT-PCR (C) The expression level of miR-124 in exosomes extracted from the BM-MSCs were determined by RT-PCR, and the protein level of CD9, CD63 and CD81 were determined by western blot (D) The expression levels of miR-124 in miR-124-exo co-cultured AsPC-1 or PANC1 cells were determined by RT-PCR (E) and cells were stained using Dil C16 dye (F). These results present the mean ± standard deviation (SD) of three independent experiments. ***P<0.001.