Figure 1. BAG5 is upregulated in DNA damage- and oxidative stress-treated cells but not in hypoxia-treated cells. (A–C) U2OS, HeLa, and SH-SY5Y cells were treated with 10 μM of etoposide for 48 h, 250 μM of H2O2 for 24 h, 10 μM of DFX for 48 h, or solvent. RNA was extracted, and BAG5 transcripts were detected by RT-Q-PCR. The relative fold in BAG5 mRNA expression was normalized to the GAPDH control and the solvent control (upper panel). The protein expression of BAG5, p53, HIF-1α, and β-actin was detected by Western blotting (lower panel). The relative fold in protein expression was normalized to that of the internal control β-actin and standardized with the solvent control. Error bars represent the SD of the means calculated using data from three independent experiments (Student’s t-test; *, p < 0.05, **, p < 0.01, ***, p < 0.001).