Research Paper Volume 12, Issue 21 pp 21854—21873

Three-gene risk model in papillary renal cell carcinoma: a robust likelihood-based survival analysis

Figure 11. Experiment of TPX2. (A) The correlation analysis between MKI67, PCNA and TPX2. (B) Immunoblot analysis of TPX2 protein in SKRC39 cells following TPX2 knockdown. GAPDH served as loading control. (C, D) EdU incorporation assays were used to determine the effects of TPX2 on SKRC39 cell proliferation. The ratio of EdU-positive cells (green) per field to the number of Hoechst 33342-positive cells (blue) in the same field was calculated in five random fields. (E) The effects of TPX2 on the proliferation of SKRC39 cells were analyzed by CCK-8 assays. The results are presented as the mean optical density (OD) at 450 nm for triplicate wells. The results are presented as the mean ± SD of three independent experiments (*P < 0.05, **P < 0.01). (F) The correlation analysis between CDH2, CTNNB1, VIM, TGFB1 and TPX2. (G, H) Knockdown of TPX2 inhibited cell migration as detected by Transwell assays. Number of cells that invaded through the membrane was counted in 10 fields under magnification, ×200; compared with NC and SKRC39 control group.