Figure 4. Relative quantitative mRNA expression through real-time PCR. The relative expression of mRNA transcripts related to (A) Wnt signaling (GSK3A, GSK3B, AXIN2, APC, DVL1, and β-Catenin), (B) antioxidant activity (CAT), and apoptosis (BAX and BCL2), are shown for the four different experimental groups: control, Klotho, LiCl, and Klotho + LiCl. The experiments were performed in triplicate, using mature oocytes from five different biological replications. Bars with letters indicate significant differences (P < 0.05).