Research Paper Volume 12, Issue 22 pp 22892—22905

USP10 deletion inhibits macrophage-derived foam cell formation and cellular-oxidized low density lipoprotein uptake by promoting the degradation of CD36

Figure 3. USP10 regulates the protein expression of CD36. (A, C) THP1 and RAW264.7 cells were exposed to Spautin-1 or USP10 siRNA. Protein was extracted and subjected to western blot for expression of CD36. (B, D) The quantitation of CD36 band were performed. (E, F) The treated cell posted with Spautin-1 was stained with FITC-labeled CD36 antibody followed by flow cytometry. (G) Cells were stained with CD36 and Cy3-conjugated antibody and subjected to confocal microscopy. *p<0.05, **p<0.01, ***p<0.001 versus each vehicle control.