Research Paper Volume 12, Issue 22 pp 22892—22905

USP10 deletion inhibits macrophage-derived foam cell formation and cellular-oxidized low density lipoprotein uptake by promoting the degradation of CD36

Figure 4. OxLDL-induced the upregulation of CD36 is reduced by USP10 inhibition. (A) Cells were treated with Spautin-1/USP10 siRNA, oxLDL or the combination of the two treatments. Protein was harvested for western blot assay to test CD36 expression. (B) The band of CD36 was counted. (C, D) The treated cells posted with Spautin-1 were stained FITC-CD36 antibody followed by flow cytometry. (E) THP1 cell was incubated with CD36 antibody and DAPI for cell nucleus. *p<0.05, ***p<0.001 versus each vehicle control.