Research Paper Volume 12, Issue 22 pp 22892—22905

USP10 deletion inhibits macrophage-derived foam cell formation and cellular-oxidized low density lipoprotein uptake by promoting the degradation of CD36

Figure 6. USP10 interacts with CD36. (A, B) Protein lysates were extracted from THP1 cells and subjected to Co-IP assay. (C) Cells were transfected with FLAG-tagged USP10 for 48 h and then stained with FLAG and CD36 antibodies, followed by confocal assay. (D, E) Cells were treated with Spautin-1 or USP10 siRNA. Immunoprecipitated with CD36 beads was subjected to immunoblotted for Ub and K48.