Research Paper Volume 12, Issue 21 pp 21874—21889

Integrated analysis of transcriptomic and metabolomic data demonstrates the significant role of pyruvate carboxylase in the progression of ovarian cancer

Figure 3. Knockdown of PC can significantly inhibit cell proliferation, cell cycle progression, and cell migration and invasion. (A) The relative expression of GGPS1, NTPCR, PPOX, PC, PRICKLE2, TCF7L1, and PPP3CA in four cancer cell lines (SKOV3, CAOV-3, OV-1063, and OVCAR-3). Gene expression levels of candidate genes (GGPS1, NTPCR, PPOX, PC, PRICKLE2, TCF7L1, and PPP3CA) were examined in different ovarian cancer cells lines using real-time qPCR analysis. (B) The relative expression of PC in SKOV-3 cell after PC knockdown using shRNAs by real-time qPCR analysis. (C) Decreased expression of PC can significantly inhibit SKOV-3 cell proliferation. (D) Cell cycle analysis of SKOV-3 cells following PC knockdown. The effect of PC on cell cycle progression was examined using flow cytometry. PC knockdown significantly inhibited cell cycle transition from G1 to S phase. (E) Cell migration and invasion of ovarian cancer cells after PC knockdown. The effects of PC on migration and invasion of SKOV3 cells were evaluated using the Transwell system. PC knockdown significantly inhibited the invasive and metastatic abilities of ovarian tumor cells.