Figure 5. MiR-338-5p rescued neuron apoptosis induced by Aβ accumulation. (A–C) Primary hippocampal neurons were isolated infected with lentiviral miR-338-5p expressing vector or lentiviral empty vector. Two days later, neurons were cultured for consecutive 3 or 7 days with or without 5 mM Aβ40. (A, B) The representative immunofluorescent images (A) and quantification (B) of TUNEL-positive hippocampal neurons in vitro. (C, D) The representative western blot images (C) and quantification analysis of cleaved caspase-3 expression (D). Scale bar=50 μm. Results are presented as mean ± SD. n = 3 in each group. *P < 0.05; **P<0.01.