Figure 1. cKD of Foxg1 in Sox9+ SCs in the mouse utricle. (A, B) Sox9 and Foxg1 mRNA (A) and protein (B) expression in P3 mouse cochlea and utricles as detected by RT-PCR and Western blotting, respectively. (C) P01 mice were i.p. injected with tamoxifen and the utricle was harvested at P3 to observe the Sox9 expression pattern in the utricle. (D) Lineage tracing showed Sox9 expression mainly in the SCs of the P3 mouse utricle. Myo7a (green) was used to indicate the HC, and Sox2 (blue) was used to label SCs. Scale bar, 10 μm. (E) Quantification of the percentage of tdTomato positive and negative cells in both the HC layer and SC layer of the ES and S regions of the utricle. (F) Foxg1 cKD efficiency was measured by qPCR. mRNA was extracted from the whole utricle. N indicates the number of real-time qPCR experimental repetitions. *p < 0.05, data are represented as mean ± SEM.