Priority Research Paper Volume 12, Issue 20 pp 19834—19851

Knockdown of Foxg1 in Sox9+ supporting cells increases the trans-differentiation of supporting cells into hair cells in the neonatal mouse utricle

Figure 5. Foxg1 cKD induced the trans-differentiation of Sox9+ SCs in the mouse utricle after neomycin injury in vitro. (A) P01 mice were i.p. injected Tamoxifen to activate the Cre enzyme, and at P3 the utricle were harvested and cultured in vitro. Neomycin (1 mM) was added to the culture medium for 12 h, and the utricle was allowed to recover for 5 days and then harvested at day 6 in vitro. DIV, days in vitro. (B) Immunofluorescence staining with anti-Myo7a (green) antibodies in the cultured utricle from Sox9CreER/+Rosa26-tdTomato and Sox9CreER/+Foxg1loxp/loxpRosa26-tdTomato mice. Myo7a was used as the HC marker. tdTomato+ HCs are indicated by white arrows. (C) Quantification of tdTomato+ HCs in the S and ES regions per 0.01 mm2 area of the utricle. (D) Quantification of the total number of HCs in the S and ES regions per 0.01 mm2 area of the utricle. Myo7a was used to indicate the HCs. Scale bar, 10 μm. “N” indicates the number of mice. *p < 0.05, ***p < 0.001; n.s., no significance, data are represented as mean ± SEM.