Liver specific activation of Akt stimulates insulin sensitivity. AMPKα and ACC signaling was measured by immunoblotting of liver, heart and skeletal muscle lysates isolated from normal chow fed (A) and high fat fed (B) Ptcd1+/+ (n=8) and Ptcd1+/- (n=8) mice. GAPDH (glyceraldehyde-3-phosphate dehydrogenase) was used as a loading control. Relative protein levels and relative intensity of phosphorylation versus total protein levels were measured using ImageJ software and normalized to GAPDH. The data are representative of results obtained from 8 mice from each genotype. Error bars are SEM. *P < 0.05, Student’s t test.