Research Paper Volume 12, Issue 21 pp 21904—21922

LINC00968 can inhibit the progression of lung adenocarcinoma through the miR-21-5p/SMAD7 signal axis

Figure 2. LINC00968 inhibits malignant phenotype in tumor cells. (A) After treatment with LINC00968 overexpressing or control plasmids, the LINC00968 expression levels were validated by qTR-PCR. (B) Cell proliferation was detected at the indicated time points. (C) Representative images of cell forming colonies and colony formation efficiency of each group. (D) The migration ability of LINC00968 overexpressing cells in each group. The histogram of the migration cell number is on the right. (E) The representative images of cells moving across the Matrigel membranes. (F) Tumor xenografts from nude mice. (G) Upregulation of LINC00968 significantly decreased tumor weight. (H) Representative images of the lung metastatic foci on the surface area. Arrows indicate the lung metastatic foci (left). The lung weights in each group are listed to the right. (I) Representative images of lung metastatic foci after HE-staining (left). The number of metastatic foci (right). *P<0.05, **P<0.01, ***P<0.001.