Figure 3. LINC00968 regulates the SMAD-mediated Hippo signaling pathway. (A) The workflow of random peptide arrays used to screen downstream proteins specifically regulated by LINC00968 in LUAD. (B) Volcano plots of the differentially expressed lncRNAs (266 upregulated and 208 down-regulated proteins) in LINC00968 overexpressing and control A549 cells. (C) The most frequent proteins and relevant signaling pathways regulated by LINC00968. (D) SMAD and STK3 expressions were increased whereas YAP1 was decreased after LINC00968 upregulation as detected by western blotting. (E) YAP1 expression (red), validated by immunofluorescence following LINC00968 overexpression in LUAD cells.