Figure 6. KIF14 silencing inhibits the AKT–ETS1–KIF14 positive feedback loop to reverse acquired resistance to sorafenib in HCC. (A and B) Sorafenib-resistant Huh7-SR and HepG2-SR cells were transfected with control or siKIF14 for 24 h and subsequently incubated with 0 or 5 μM sorafenib for 24 h. The protein expression profiles of ETS1, AKT, and p-AKT were detected by western blotting (A). The density of each band was normalized to that of β-actin (B). (C) Control or siKIF14-transfected Huh7-SR cells were stained with anti-KIF14 Ab (red), anti-p-Akt Ab (green), and DAPI (cellular nuclei, blue) and viewed with an inverted fluorescence microscope. Data represent three independent experiments. Scale bar = 10 μm. NS, not significant. “*” Indicates P<0.05 and “**” indicates P<0.001.