Research Paper Volume 12, Issue 22 pp 22975—23003

Silencing KIF14 reverses acquired resistance to sorafenib in hepatocellular carcinoma

Figure 6. KIF14 silencing inhibits the AKT–ETS1–KIF14 positive feedback loop to reverse acquired resistance to sorafenib in HCC. (A and B) Sorafenib-resistant Huh7-SR and HepG2-SR cells were transfected with control or siKIF14 for 24 h and subsequently incubated with 0 or 5 μM sorafenib for 24 h. The protein expression profiles of ETS1, AKT, and p-AKT were detected by western blotting (A). The density of each band was normalized to that of β-actin (B). (C) Control or siKIF14-transfected Huh7-SR cells were stained with anti-KIF14 Ab (red), anti-p-Akt Ab (green), and DAPI (cellular nuclei, blue) and viewed with an inverted fluorescence microscope. Data represent three independent experiments. Scale bar = 10 μm. NS, not significant. “*” Indicates P<0.05 and “**” indicates P<0.001.