Figure 1. GPR30 protein accumulated in COCs after LH/hCG injection. PMSG-primed female mice were injected with hCG, and the COCs in the ovaries were collected at 0, 2, 4, 8, and 16 h. The mRNA levels of GPR30 were measured using RT-qPCR. (A) The protein levels of GPR30 were detected using western blot (B), and the bands were quantified using gray scanning (C). Data are represented as fold induction relative to the unstimulated control (0 h). The bars of panels A and C are represented as average±SEM. Different lowercase letters indicate significant differences among the different groups (p<0.05). Three independent replicates were performed for each experiment.