Figure 4. Illustration of mice treatment. In the control group, mice were intraperitoneally injected with captisol (dissolved in saline) on days 1, 3, 5, and 7, and PMSG was injected on day 5. Saline was injected on day 7, COCs were collected on day 8. In the hCG group, all treatments were the same as for the control group, except that hCG was injected in mice instead of saline. In the AG1478 group, mice were treated with AG1478 on days 1, 3, 5, and 7, and PMSG was injected on day 5. hCG was then injected on day 7, and COCs were collected on day 8.