Research Paper Volume 12, Issue 20 pp 20801—20816

Synergistic effect between LH and estrogen in the acceleration of cumulus expansion via GPR30 and EGFR pathways


The GPR30 protein is degraded via the lysosomal pathway. FGCs were treated with the lysosome inhibitor BA1 or proteasome inhibitor MG132 (MG) for 4 h, GPR30 was detected using Western blot (A), and the quantification of GPR30 protein levels are shown in the diagram (B). Data are presented as fold induction relative to the unstimulated control. Bars are presented as the average±SEM. Different lowercase letters indicate significant differences between groups (p<0.05). Three independent replicates were performed for each experiment.