Research Paper Volume 12, Issue 21 pp 21971—21991

CircGLCE alleviates intervertebral disc degeneration by regulating apoptosis and matrix degradation through the targeting of miR-587/STAP1

Figure 4. Silencing miR-587 reverses disc degeneration induced by CircGLCE downregulation. After transfecting NP cells with sh-CircGLCE, the combined sh-CircGLCE and miR-587 sponge adenovirus, or the control, (A) the levels of ECM markers were measured with western blotting (n=3); (B) the levels of ECM markers were measured with FISH; (C) the expression levels of ECM markers were measured with RT-qPCR (n=3, ***P < 0.001, one-way ANOVA coupled with Tukey’s post hoc test). Data are the mean ± SEM.