Figure 2. SEMA4C mRNA and protein levels are upregulated in the CRC tissues. (A) The relative SEMA4C mRNA expression level in normal colorectal and CRC tissues from the TCGA-COAD and TCGA-READ datasets. (B) Representative micrographs show SEMA4C immunohistochemical staining of the 83 pairs of colon cancer and adjacent normal colon tissue samples in the tissue microarray (TMA). Note: Scale bars=200 μm; red arrow indicates strong positive SEMA4C protein expression in the colon cancer tissue; blue arrow indicates negative SEMA4C protein expression in the adjacent normal tissue. (C) Quantitative analysis of SEMA4C protein expression scores based on the immunohistochemical staining of the TMA with 83 pairs of colon carcinoma and adjacent normal colon tissue samples.