Research Paper Volume 12, Issue 22 pp 23017—23028

Autophagy is induced in human keratinocytes during human papillomavirus 11 pseudovirion entry

Figure 2. LC3 puncta are increased after HPV11 PsV infection of NHEK cells. (A) Confocal microscopy analysis of GFP-LC3 puncta and L1-stained HPV11 PsVs. The NHEK nucleus was stained with 4’,6-diamidino-2-phenylindole (DAPI). (B) Quantification of GFP-LC3 puncta in panel (A). (C) Quantification of the total L1 staining area in panel (A). The graphs represent the mean and standard deviation of three biological independent experiments, and at least 100 cells were analyzed for each repeat. Significant differences were identified by Student’s t test. *, P < 0.05.