Figure 2. Effect of APG treatment on phosphorylation of GSK-3β at Ser-9 and nuclear Nrf2 expression. (A) Comprehensive photograph of p-GSK-3β (Ser-9), total GSK-3β, and nuclear Nrf2 expression and the corresponding β-actin and histone-3 bands. (B) APG treatment increased the phosphorylation of GSK-3β compared with the OGD group. (C) Statistical analysis of western blotting result for Nrf2 in the control, OGD, and APG (2 μM, 4 μM and 8 μM) treatment groups. APG treatment increased Nrf2 nuclear expression compared with the OGD group. n = 5 per group. * P < 0.05, ** P < 0.01, *** P < 0.001. NS = not significant.