Research Paper Advance Articles pp 23872—23888

Apigenin-7-O-β-D-(-6”-p-coumaroyl)-glucopyranoside treatment elicits a neuroprotective effect through GSK-3β phosphorylation-mediated Nrf2 activation

Figure 3. Knockdown of Nrf2 via RNA interference prevented the nuclear Nrf2 translocation and protective effect induced by APG. (A) The upper portion shows a comprehensive photograph of Nrf2 and the corresponding histone-3 bands. The lower portion shows Nrf2 expression in the presence of Nrf2-siRNA or Nrf2-siRNAc. (B) Effect of Nrf2-siRNA or Nrf2-siRNAc on plasma lactate dehydrogenase (LDH) release level in primary cortical neuron cultures treated with APG. (C) Effect of Nrf2 knockdown on cell viability in primary cortical neuron cultures with APG treatment. n = 5 per group. * P < 0.05, ** P < 0.01, *** P < 0.001.