Research Paper Advance Articles pp 23872—23888

Apigenin-7-O-β-D-(-6”-p-coumaroyl)-glucopyranoside treatment elicits a neuroprotective effect through GSK-3β phosphorylation-mediated Nrf2 activation

Figure 8. PI3K inhibitors wortmannin and LY294002 or Nrf2 knockdown abated the increase in antioxidant enzymes and reversed the relief of oxidative stress induced by APG treatment. Cellular oxidative products and antioxidant enzyme activities are illustrated. Both the GSK-3β phosphorylation inhibition induced by PI3K inhibitors and the Nrf2 knockdown produced by siRNA increased the 8-OHdG (A), 4-HNE (B) and ROS (C) contents and decreased all the content of SOD (D), CAT (E) and GSH-px (F) (n =6 per group). ** P < 0.01, *** P < 0.001 vs. I/R+APG group.