Figure 3. (A, B) Western blots of proteins related to senescence and inflammation at PID 15 in shNegative, shSirt1, and shSirt1+LKB1 K48R cells. (A) The aforementioned proteins were assessed by western blot in PID 15 shNegative, Sirt1 knockdown, and Sirt1 knockdown + LKB1 K48R expressing 3T3-L1 cells. Upregulation of cyclin kinase inhibitor p21CIP, inflammatory chemokine receptor IL8b, and TGF-beta was observed in Sirt1 knockdown cells, and LKB1 K48R prevented these changes. (n=4, *p<0.05 vs. shNegative).