Research Paper Volume 12, Issue 19 pp 19766—19784
Autism-like behaviors and abnormality of glucose metabolism in offspring derived from aging males with epigenetically modified sperm
Body weight, blood glucose during GTT and insulin concentrations in F2 progeny. (A) the body weight trajectories in male F2 progeny (n=30 male offspring for each group, ≥ 5 litter size/group). (B) Fasting blood glucose. (n=15 male offspring for each group, ≥ 5 litter size/group). (C–F) Blood glucose during GTT at 6 (C and D) and 8 (E and F) weeks of age (n=15/each sexual offspring, ≥ 5 litter size/group). (G) Plasma insulin concentration in glucose-stimulated conditions (n=15/male offspring for each group, ≥ 5 litter size/group). mF2 and fF2 were referred to the male and female progenies from F1 mice, respectively. Data are presented as the mean ± s.e.m. NS, P≥0.05; ***, P<0.001, versus control (linear regression analysis for GTT assay and one-way ANOVA for the body weight trajectories, fasting blood glucose and AUC results).