Research Paper Volume 12, Issue 22 pp 23114—23128

Intracellular and extracellular S100A9 trigger epithelial-mesenchymal transition and promote the invasive phenotype of pituitary adenoma through activation of AKT1

Figure 4. Effects of intracellular S100A9 on proliferation, migration and invasion in PA. (A) HP75 cell viability in control group (group 1), NC1 group (group 2), LV-S100A9 group (group 3) and LV-S100A9 + A-674563 group (group 4) was tested using CCK-8 after 24, 48, 72 and 96h. (B) The cell cycle distribution was determined by FCM in each of the four groups (groups 1, 2, 3, 4) after 96h. (C) Wound-healing assay and transwell system were used to measure the migration and invasion of HP75 cells in the four groups (groups 1, 2, 3, 4). (n=5. *P<0.05; **P<0.01; ***P<0.001, compared with group 1. #P<0.05; ##P<0.01; ###P<0.001, compared with group 4). (D) CCK-8 to observe the proliferation of HP75 cells in control group (group a), NC2 group (group b), S100A9-shRNA-LV group (group c) and S100A9-shRNA-LV + SC79 group (group d) for 24, 48, 72 and 96h. (E) The cell cycles were estimated in group a, b, c, and d using FCM. (F) The migration and invasion were also investigated using wound-healing assay and transwell system in groups a, b, c, and d. (n=5. *P<0.05; **P<0.01; ***P<0.001 vs. group a. #P<0.05; ##P<0.01; ###P<0.001vs. group d).