Figure 4. Effects of intracellular S100A9 on proliferation, migration and invasion in PA. (A) HP75 cell viability in control group (group 1), NC1 group (group 2), LV-S100A9 group (group 3) and LV-S100A9 + A-674563 group (group 4) was tested using CCK-8 after 24, 48, 72 and 96h. (B) The cell cycle distribution was determined by FCM in each of the four groups (groups 1, 2, 3, 4) after 96h. (C) Wound-healing assay and transwell system were used to measure the migration and invasion of HP75 cells in the four groups (groups 1, 2, 3, 4). (n=5. *P<0.05; **P<0.01; ***P<0.001, compared with group 1. #P<0.05; ##P<0.01; ###P<0.001, compared with group 4). (D) CCK-8 to observe the proliferation of HP75 cells in control group (group a), NC2 group (group b), S100A9-shRNA-LV group (group c) and S100A9-shRNA-LV + SC79 group (group d) for 24, 48, 72 and 96h. (E) The cell cycles were estimated in group a, b, c, and d using FCM. (F) The migration and invasion were also investigated using wound-healing assay and transwell system in groups a, b, c, and d. (n=5. *P<0.05; **P<0.01; ***P<0.001 vs. group a. #P<0.05; ##P<0.01; ###P<0.001vs. group d).