Research Paper Advance Articles pp 23974—23995

Effect of lncRNA WT1-AS regulating WT1 on oxidative stress injury and apoptosis of neurons in Alzheimer's disease via inhibition of the miR-375/SIX4 axis

Figure 1. WT1-AS inhibited OSI and apoptosis of SH-SY5Y cells treated with Aβ25-35 (A) The two circles in the figure represent the differential expression of lncRNAs in GSE4757 and lncRNAs related to AD obtained from the LINCDISEASE database, respectively, and the middle part represents the intersection of the two datasets. (B) WT1-AS expression in GSE4757; abscissa, sample type; ordinate, gene expression; blue box, normal sample; and red box, tumor sample. (C) The expression of WT1-AS measured by qRT-PCR. (D) The expression of p-Tau and total Tau detected by western blot. (E) Detection of mitochondrial membrane potential by JC-1 staining (200x). (F) Detection of ATP content. (G) Detection of ROS content. (H) Detection of MDA content, SOD and GSH-Px activities. (I) Detection of LDH activity. (J) Detection of apoptosis by flow cytometry. *P<0.05; the experimental results are expressed as the mean ± standard deviation. Differences among multiple groups were analyzed using one-way ANOVA followed by Tukey’s post hoc test. The experiment was repeated three times.